sapidus reovirus 1 (CsRV1, also called RLV for reo-like virus), throughout the studied US range from Louisiana to Massachusetts ( Johnson, 1977 Bowers et al., 2010 Rogers et al., 2014 Flowers et al., 2015). Blue crabs are infected by a pathogenic virus, C. Blue crab populations are themselves subject to control by numerous factors, including predation and disease ( Johnson, 1977 Hines, 2007 Shields and Overstreet, 2007 Schott and Messick, 2010 van Montfrans et al., 2010). sapidus is an adaptable euryhaline predator and scavenger that can influence and regulate benthic community structure ( Arnold, 1984 Lipcius and Hines, 1986 Baird and Ulanowicz, 1989 Hines, 2007). Anecdotal data suggest that Brazil’s long coastline has the potential for large harvests, particularly in the south ( Mendonça et al., 2010). Though harvested mostly in artisanal fisheries, landings are substantial: annual harvests average over 77,000 tonnes in the USA, 8,100 tonnes in Mexico, and 11,500 tonnes in Venezuela, ( NOAA National Marine Fisheries Service and Commercial Fisheries Statistics, 2015 Perry and VanderKooy, 2015 Singh-Renton and McIvor, 2015). The blue crab, Callinectes sapidus, plays crucial roles in the economies and ecosystems of the Atlantic coasts of North and South America ( Williams, 1974). sapidus populations across the Northern and Southern hemispheres. The sequencing, annotation, and preliminary population metrics of the genome of CsRV1 should facilitate additional studies in diverse disciplines, including structure-function relationships of reovirus proteins, investigations into the evolution of the Reoviridae, and biogeographic research on the connectivity of C. Pairwise analysis of predicted protein sequences shows that CsRV1 strains in Brazil can be distinguished from those in North America based on conserved residues in this gene. An 860 nucleotide region of the CsRV1 RdRP gene was amplified and sequenced from 15 infected crabs collected from across the geographic range of C. A region of 98% nucleotide sequence identity between CsRV1 and the only available sequence of the P virus of Macropipus depurator suggests that these two viruses may be closely related. Comparison of the CsRV1 RNA-dependent RNA polymerase (RdRP) sequence to genomes of other crab-infecting reoviruses shows that it is similar to the mud crab reovirus found in Scylla serrata and WX-2012 in Eriocheir sinensis, Chinese mitten crab, and supports the idea that there is a distinct “Crabreo” genus, different from Seadornavirus and Cardoreovirus, the two closest genera in the Reoviridae. Putative functions could be assigned for 3 of the 13 proteins encoded in the genome, based on their similarity to proteins encoded in other reovirus genomes. The 12 genome segments of a North American strain of CsRV1 were sequenced using Ion Torrent technology. sapidus Reovirus 1 (CsRV1), a double stranded RNA virus. The blue crab, Callinectes sapidus Rathbun, 1896, which is a commercially important trophic link in coastal ecosystems of the western Atlantic, is infected in both North and South America by C. 6Department of Biology, Morgan State University, Baltimore, MD, USA.5Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.4National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, USA.3National Veterinary Services Laboratories, Animal and Plant Health Inspection Service, United States Department of Agriculture, Ames, IA, USA.2University of Maryland School of Medicine, Baltimore, MD, USA.1Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, MD, USA. ![]() Vinagre 5, Shanai Brown 6, Andréa Santos e Almeida 1 and Eric J.
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